OKUNO,Yota - Faculty of Chemistry, Materials and Bioengineering Department of Chemistry and Materials Engineering

OKUNO, Yota

Profile
Research Activities
Research Publications
Community Service
Courses

Faculty, Department/Institute

Faculty of Chemistry, Materials and Bioengineering Department of Chemistry and Materials Engineering

Academic status (qualification)

Assistant Professor Apr. 1,2022

Undergraduate Degrees・University

Kyoto University Faculty of Engineering2015

Graduate Degrees・University

Kyoto University Master's Degree Program 2017

Academic Degrees

Mar. 2022

Homepage Address, E-mail Address

E-mail Address：y_okuno@kansai-u.ac.jp

Research fields

Research fields	keyword
Fabrication of Nanomaterials by self-organization method and their fucntionalization
Nanomaterials/Nanobioscience
Nanoparticle technology
Microdevices/Nanodevices
Chemistry of amino acids
Polysaccharide chemistry
Bio-relevant material chemistry
Biomolecular chemistry
Chemistry related to living body
Polymer chemistry
Polymer materials

Research topics

research topic
Study theme state	Individual Research
research duration	2023 ～ 2025
Research Programs	Grant-in-Aid for Scientific Research
keyword	,,
Research field	Fabrication of Nanomaterials by self-organization method and their fucntionalization
Research Topics Overview

Awards

Nov. 11,2021
2022 Popular Advances collection Jan. 13,2023

Intellectual Property Rights

application number：特開2023-061846
application number：特開2022-129658
application number：特開2022-001432
application number：特開2021-091861
application number：特願2021-099242
application number：特願2021-028424
application number：特願2022-074619
application number：特願2020-567627
application number：特願2020-056222
application number：特開2020-100846
application number：特許第6780795号
application number：特願2020-119994
application number：特願2020-106813
application number：WO2020-095702
application number：特願2019-561328
application number：特許第6702516号
application number：特開2020-007529
application number：特願2020-009350
application number：特願2019-10126
application number：特願2018-124523
application number：特願2023-132597 (Aug. 16,2023)

Research Publications

No.	Type of publication	Date of publication (Date of presentation)	Title	Type of research result	Jointly authored or single authored	Publisher and journal name	Volume number
1	Papers1	2023/12/22～2023/12/222023,12,22,2023,12,22	Encapsulation of multiple enzymes within a microgel via water-in-water emulsions for enzymatic cascade reactions	Academic Journal	Co-author	Royal Society of Chemistry, Soft Matter

PapersEncapsulation of multiple enzymes within a microgel via water-in-water emulsions for enzymatic cascade reactionsIn refereedAcademic JournalCo-authorOKUNO,Yota;;;Royal Society of Chemistry, Soft Matter2023/12/22～2023/12/22Enzyme-loaded spherical microgels with diameters of several micrometers have been explored for use in therapeutic microreactors and biosensors. Conventional preparation strategies for enzyme-loaded microgels utilized water-in-oil emulsions or flow chemistry techniques. The former damage enzyme activity using organic solvents and the latter are expensive and difficult to expand because of the complex system. In this study, we present a simple strategy for creating multiple enzyme-loaded gelatin-based microgels with tunable diameters in a single flask. This strategy was based on our finding that enzymes spontaneously partitioned in a dispersed methacryloyl gelatin aqueous solution in a poly(vinylpyrrolidone) (WGelMA/WPVP) aqueous solution. The method achieved an encapsulation efficiency of over 70% even with four types of enzymes and retained their activity owing to the full aqueous system. Additionally, the encapsulated β-galactosidase activity was maintained for 24 hours at pH 6, although naked β-galactosidase lost approximately 60% of its activity, which was superior to that of previous enzyme-loaded gelatin gels. Moreover, this simple method enabled the production of 10 g-scale or more microgels in one batch. We also demonstrated that multiple enzyme-loaded gelatin microgels functioned as cascade microreactors for lactose and glucose sensing. This versatile strategy enables the production of enzyme-loaded microgels while maintaining the enzyme activity using very low technologies. This result contributes to the easy preparation of enzyme-loaded microgels and their applications in the biomedical and green catalytic fields.

Academic presentationUnrefereedOKUNO,Yota2023/7/29～2023/7/29

Academic presentationUnrefereedOKUNO,Yota2023/7/18～2023/7/20

Academic presentationUnrefereedOKUNO,Yota2023/7/14～2023/7/14

International academic conferenceUnrefereedOKUNO,Yota2023/7～2023/7

Academic presentationUnrefereedOKUNO,Yota2023/5/24～2023/5/26

PapersIn refereedAcademic JournalCo-authorOKUNO,Yota2023/5/16～2023/5/16

Academic presentationUnrefereedOKUNO,Yota2023/3/8～2023/3/10

Academic presentationUnrefereedOKUNO,Yota2023/3/2～2023/3/2

Academic presentationUnrefereedOKUNO,Yota2022/12/5～2022/12/7

Academic presentationUnrefereedOKUNO,Yota2022/11/22～2022/11/22

Academic presentationUnrefereedOKUNO,Yota2022/11/21～2022/11/22

Academic presentationUnrefereedOKUNO,Yota2022/11/14～2022/11/15

Academic presentationUnrefereedOKUNO,Yota2022/9/5～2022/9/5

Magazine articleUnrefereedAcademic JournalSingle-AuthorOKUNO,Yota2022/9～2022/9

CommentaryUnrefereedAcademic JournalSingle-AuthorOKUNO,Yota2022/8～2022/8

PapersIn refereedAcademic JournalCo-authorOKUNO,Yota2022/6/27～2022/6/27

Magazine articleUnrefereedAcademic JournalCo-authorOKUNO,Yota2022/4～2022/4

BookUnrefereedMonographCo-authored chapterOKUNO,Yota2022/2～2022/2

Academic presentationUnrefereedOKUNO,Yota2021/11/28～2021/11/30

Academic presentationUnrefereedOKUNO,Yota2021/11/10～2021/11/11

International academic conferenceOKUNO,Yota2021/11～2022/11

Academic presentationUnrefereedOKUNO,Yota2021/9/6～2021/9/8

PapersIn refereedAcademic JournalCo-authorOKUNO,Yota2021/6/24～2021/6/24

Academic presentationUnrefereedOKUNO,Yota2021/5/26～2021/5/28

Academic presentationUnrefereedOKUNO,Yota2020/7/10～2020/7/10

PapersIn refereedAcademic JournalCo-authorOKUNO,Yota2019/12/30～2019/12/30

Academic presentationUnrefereedOKUNO,Yota2016/11/9～2016/11/10

Academic presentationUnrefereedOKUNO,Yota2016/9/14～2016/9/16

Foreign Languages

Foreign language presenting research result at international conference:英語
Foreign language preparing written thesis for presentation at international conference:英語

Courses Taught

Experiments of Chemistry
Freshmen Seminar
Experiments of Fundamental Chemistry
Exercises in Thesis Projects I and II
Thesis Projects I
Thesis Projects II

OKUNO, Yota